Effect of savory essential oil, garlic powder, and garlic aqueous extract on fungal load of poultry feed

Document Type: Original Articles


1 Associate Professor of Microbiology Department, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

2 Graduate of Veterinary Medicine, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

3 PhD student in microbiology, Faculty of Veterinary Medicine, Urmia University,Urmia, Iran


In this study, we investigated the effect of savory (Satureja hortensis) essential oil, garlic
(Allium sativum) powder, and garlic aqueous extract on fungal load of poultry feed. To
this end, savory essential oil and garlic aqueous extract each amounting 62.5, 125, and
250 μl/ml and mg/ml, respectively, per kilogram of feed and garlic powder amounting
2.5, 5, and 10 g/kg of feed were used. One and two weeks after adding the desired
amounts of these compounds to poultry feed, old counting was carried out at 2, 24, 48,
and 72 h. Then, the results of mold counting per gram of feed were evaluated in the
treatment groups compared to the control group. Based on results, fungal load of feed was
reduced 2 h after adding savory essential oil, and this effect was sustained until the end of
the second week. This effect was concentration-dependent, and hence fungal load reached
to zero at the highest concentration (250 μl/ml) 48 and 72 h after adding savory essential
oil (P< 0/05). Garlic powder significantly reduced the fungal load of feed, especially in
the treatment group containing the maximum dose (10 g) 72 h after adding garlic powder
(P< 0.05). The garlic aqueous extract increased fungal load of feed considerably (P<
0.05). So, it is suggested that savory essential oil and garlic powder can be used as
appropriate alternatives for chemical agents used in controlling fungal load of poultry


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