Determination of aflatoxin M1 levels in raw milk samples using ELISA and high-performance liquid chromatography in Qazvin, Iran

Document Type : Original Articles


1 Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

2 Department of Food Hygiene and Aquatics, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

3 Laboratory of Food Quality Control and Hygiene, Veterinary Medicine Office, Qazvin, Iran

4 Department of Pathobiology, Division of Mycology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran


Use of raw milk and dairy products containing aflatoxin M1 (AFM1) has led to concern in
consumers. The present study determined the AFM1 in raw cow's milk in Qazvin
province. In this research, 170 raw cow's milk were collected from dairy farm, dairy
factories, milk collection centers, and milk supply centers in Qazvin province during cold
seasons in 2013, and all samples were examined for AFM1. The samples were analyzed
with a commercial competitive enzyme-linked immunosorbent assay (ELISA) kit and
high-performance liquid chromatography (HPLC). AFM1 contamination was observed in
all milk samples. Fifty-seven milk samples (33.52%) had a contamination of AFM1,
higher than the threshold level of The Institute of Standards and Industrial Research of
Iran (0.5 ng/ml), whereas in 113 milk samples (66.48%), concentration of AFM1 was less
than the limits permitted. The mean concentration of AFM1 in dairy farm was 0.215
ng/ml, in dairy factories 0.268 ng/ml, in milk collection centers 0.734 ng/ml, and in milk
supply centers 0.409 ng/ml. Because of high levels of contamination observed in samples,
regular monitoring of contamination in milk samples and controlling most contaminating
causes are necessary.


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