Identification and antifungal susceptibility pattern of Candida species isolated from patients with nosocomial candiduria

Document Type : Original Articles

Authors

1 Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

2 Hashemi Nejad Hospital, Iran University of Medical Sciences, Tehran, Iran

3 Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

4 Department of Microbiology and Immunology, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran

Abstract

Introduction: Nosocomial candiduria could be due to cystitis, pyelonephritis, or fungus
ball in the urinary tract system. Several reports have shown candidemia and upper urinary
tract involvement as the complications of candiduria. The aim of this study was to assess
nosocomial candiduria; identify Candida isolates and determine their drug susceptibility
pattern. Materials and Methods: Urine samples of 115 hospitalized patients were
collected during a period of five months. Candida species were isolated and identified
using conventional and molecular (PCR-RFLP) diagnostic methods. Antifungal
susceptibility profiles for amphotericin B and fluconazole were performed using broth
microdilution method, based on the Clinical and Laboratory Standards Institute (CLSI)
M27-A2 guideline. Results: Nosocomial candiduria was diagnosed in 5 (4.3%) patients.
The isolated Candida species were identified as Candida albicans (n: 4) and C. glabrata
(n: 2). Two strains of C. albicans, and C. glabrata were resistant to fluconazole.
Conclusion: Similar to several reports, the results of this study show that C. albicans is
the main Candida species causing nosocomial candiduria and drug resistant Candida
species are causative agents of candiduria in hospitalized patients.

Keywords

References

1. Achkar, J.M., Fries, B.C., 2010. Candida infection of the Genitourinary Tract. Clin Microbiol Rev,23: 253-273.
2. Almeida, A.A., Nakamura, SS., Fiorini, A., Grisolia, AB., Svidzinski, TI., Oliveira, K.M., 2015. Genotypic variability and antifungal susceptibility of Candida tropicalis isolated from patients with candiduria. Rev Iberoam Micol, 32(3): 153-158.
3. Bakhary, A.Z., 2008. Candiduria: A review of clinical significance and manaegement. Saudi Kidny Dis Transpl, 19(3): 350-360.
4. Behzadi, P., Behzadi, E., Ranjbar, R., 2015. Urinary tract infection and Candida albicans. Cent European J Urol, 68(1): 96-101.
5. Calderone, RA., 2002. Candida and candidiasis. American Society Microbiology (ASM) press, USA.
6. de Freitas, A.R., Baeza, L.C., Faria, M.G., Dota, K.F., Godoy Martínez, P., Svidzinski, T.I., 2014. Yeast isolated from nosocomial urinary infections: antifungal susceptibility and biofilm production. Rev Iberoam Micol, 31(2): 104-108.
7. Dismukes, W., Pappas, P.G., Sobel, J.D., 2003. Clinical Mycology. Oxford University press, USA.
8. Fakour, F., Falahati, M., Zaini, F., Mousavi Nasab, N., 2004. A survey of candiduria in diabetic patients of Zanjan, 2001-2002. Iran Uni Med Sci J, 11(41): 453-462.
9. Fraisse, T., Crouzet, J., Lachaud, L., Durand, A., Charachon, S., Lavigne, J.P., et al., 2011. Candiduria in those over 85 years old: a retrospective study of 73 patients. Intern Med, 50(18): 1935–1940.
10. Goetz, L.L., Howard, M., Cipher, D., Revankar, S.G., 2010. Occurrence of candiduria in population of chronically catheterized patients with spinal cord injury. Spinal Cord, 48(1): 51-54.
11. Guler, S., Ural, O., Findik, D, Arslan, U.,
2006. Risk factor for nosocomial candiduria. Saudi Med J, 27(11): 1706-1710.
12. Joz Panahi, M., Mobin, A., Karami, A., Ahadi, S., 2011. Frequency of candiduria in patients hospitalized in intensive care units. J Kerman Uni Med Sci, 18(3): 228-234.
13. Kauffman, C.A., Vazquez, J.A., Soble, J.D., Gallis, H.A., Mckinsey, D.S., Karchmer, A.W., Sugar, A.M., et al., 2000. Prospective multicenter surveillance study of funguria in hospitalized patients. Clin Infect Dis, 30(1): 14-18.
14. Kauffman, C.A.,2005. Candiduria. Clin Infect Dis, 41(6): 371-376.
15. Lundstrom, T., Sobel, J.D., 2001. Nosocomial candiduria. Clin Infect Dis, 32: 1602-1607.
16. Nayman Alpat, S., Ozgunes, I., Ertem, O.T., Erben, N., Doyuk Kartal, E., Tozun, M., Usluer, G., 2011. Evaluation of risk factors in patients with candiduria. Mikrobiyol Bul, 45(2): 318-324.
17. Ozhak–Baysan, B., Ogunc, D., Colak, D., Ongut, G., Donme,z ., Vura,l T., Gunseren, F., 2012. Distribution and antifungal susceptibility of Candida species causing nosocomial candiduria. Med Mycol, 50: 529–532.
18. Padawer, D., Pastukh, N., Nitzan, O., Labay, K., Aharon, I., Brodsky, D., Glyatman, T., Peretz, A., 2015. Catheter-Associated candiduria: Risk factors, medical interventions and antifungal susceptibility. Am J Infect Control, 43(7): e 19-22.
19. Pakshir, K., Moghaddami, M., Emmami, M., Kordbacheh, P., 2004. Prevalence and Identification of Etiological Agents of Funguria in Foley Catheterized Patients. J Med Res, 2(3): 33-41.
20. Seifi, Z., Azish, M., Salehi, Z.,Zarei Mahmoudabadi, A., Shamsizadeh, A., 2013.Candiduria in children and susceptibility patterns of recovered Candida species to antifungal drugs in Ahvaz. J Nephropathol, 2(2): 122-128. 
21. Sellami, A., Sellami, H., Makni, F., Bahloul, M., Cheikh-Rouhou, F., Bouaziz, M., Ayadi, A., 2006. Candiduria in intensive care unit: significance and value of yeast numeration in urine. Ann Fr Anesh Reanim,25: 584-588.
22. Singla, N., Gulati, N., Kaistha, N., Chander, J., 2012. Candiduria colozination in urine samples of ICU patients: determination of etiology antifungal susceptibility testing and evaluation of associated risk factor. Mycopathologia, 174(2): 149-155.
23. Yashavanth, R., Shiju, MP., Bhaskar, UA., Ronald, R., Anita, KB., 2013. Candiduria: Prevalence and Trends in Antifungal Susceptibility in A Tertiary Care Hospital of Mangalore. J Clin Daign Res, 7(11): 2459-2461.
24. Zaini, F., Azordegan, F., Chabavizadeh, J., 1993. Study of fungal infections in urine. Iranian Journal of Public Health, 4(22): 13-31.
25. Zarei Mahmoudabadi, A., Zarrin, M., Ghanatir, F., Vazirianzadeh, B., 2012. Candiduria in hospitalized patients in teaching hospitals of Ahvaz. Iranian Journal of Microbiology, 4(4): 198- 203.
26. Zarei Mahmoudabadi, A., Zarrin, M., Fard, M.B., 2013. Antifungal Susceptibility of Candida species isolated from candiduria. Iranian Journal of Microbiology, 6(1): 24-28.
Journal of Mycology Research
Volume 2, Issue 2 - Serial Number 2
Summer & Autumn
2015
Pages 77-84

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