Identification and antifungal susceptibility pattern of Candida species isolated from patients with nosocomial candiduria

Document Type: Original Articles

Authors

1 Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

2 Hashemi Nejad Hospital, Iran University of Medical Sciences, Tehran, Iran

3 Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

4 Department of Microbiology and Immunology, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran

Abstract

Introduction: Nosocomial candiduria could be due to cystitis, pyelonephritis, or fungus
ball in the urinary tract system. Several reports have shown candidemia and upper urinary
tract involvement as the complications of candiduria. The aim of this study was to assess
nosocomial candiduria; identify Candida isolates and determine their drug susceptibility
pattern. Materials and Methods: Urine samples of 115 hospitalized patients were
collected during a period of five months. Candida species were isolated and identified
using conventional and molecular (PCR-RFLP) diagnostic methods. Antifungal
susceptibility profiles for amphotericin B and fluconazole were performed using broth
microdilution method, based on the Clinical and Laboratory Standards Institute (CLSI)
M27-A2 guideline. Results: Nosocomial candiduria was diagnosed in 5 (4.3%) patients.
The isolated Candida species were identified as Candida albicans (n: 4) and C. glabrata
(n: 2). Two strains of C. albicans, and C. glabrata were resistant to fluconazole.
Conclusion: Similar to several reports, the results of this study show that C. albicans is
the main Candida species causing nosocomial candiduria and drug resistant Candida
species are causative agents of candiduria in hospitalized patients.

Keywords


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