Phylogenetic analysis of HSP70 gene of Aspergillus fumigatus reveals conservation intra-species and divergence inter-species

Document Type : Original Articles

Authors

1 Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

3 Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

Abstract

Aspergillus fumigatus is a saprophyte fungus, widely spread in a variety of ecological
niches and the most prevalent aspergilli responsible for human and animal invasive
aspergillosis. The first step to develop novel and efficient therapies is the identification
and understanding of the key tolerance and virulence factors of pathogens. The main
focus of the present study is to perform the similarity, conservation and phylogenetic
analysis of heat shock protein 70 (HSP70) gene of the fungus A. fumigatus. Therefore,
DNA sequence of HSP70 gene was obtained from a native airborne A. fumigatus.
Similarity, divergence and conservation of HSP70 gene sequence were assayed and
compared to other strains and fungal species using nucleotide Basic Local Alignment
Search Tool (BLAST), construction of phylogenetic tree and online analysis tools. The
results revealed that the most similar aspergilli to the examined A. fumigatus strain were
A. fumigatus non-native strains, Aspergillus clavatus, Aspergillus niger, Aspergillus
terreus, and Aspergillus nidulans. Among other fungal genera, Penicillium species were
the most similar fungal genus to A. fumigatus. Moreover, the alignment results revealed
high levels of similarity and conservation of native A. fumigatus HSP70 gene sequence
with yeasts stress-Seventy subfamily A (SSA) gene sequence from HSP70 family of
genes and lower similarities with other genes in the family (Ssb, Ssc, HscA, Kar2 and
bipA). Penicillium spp. were the most similar fungi to Aspergillus species by
phylogenetic analysis of HSP70 sequence. Other molds, yeasts and yeast-like fungi were
placed in more distanced clades. In general, fungal HSP70 sequence analysis could direct
us to make a better understanding of thermo-tolerance in different fungal species.

Keywords

References

1. Allendoerfer, R., Maresca, B. and Deepe, G., 1996. Cellular immune responses to recombinant heat shock protein 70 from histoplasma capsulatum. Infection and immunity, 64:4123-4128.
2. Arnaud, M.B., Chibucos, M.C., Costanzo, M.C., Crabtree, J., Inglis, D.O., Lotia, A., Orvis, J., Shah, P., Skrzypek, M.S., Binkley, G., Miyasato, S.R., Wortman, J.R. and Sherlock, G., 2010. The aspergillus genome database, a curated comparative genomics resource for gene, protein and sequence information for the aspergillus research community. Nucleic Acids Res, 38:D420-427.
3. Ashkenazy, H., Erez, E., Martz, E., Pupko, T. and Ben-Tal, N., 2010. Consurf 2010: Calculating evolutionary conservation in sequence and structure of proteins and nucleic acids. Nucleic Acids Res, 38:W529-533.
4. Beffa, T., Staib, F., Lott Fischer, J., Lyon, P., Gumowski, P., Marfenina, O., Dunoyer-Geindre, S., Georgen, F., Roch-Susuki, R. and Gallaz, L., 1998. Mycological control and surveillance of biological waste and compost. Medical Mycology, 36:137-145.
5. Beffa, T., Staib, F., Lott Fischer, J., Lyon, P.F., Gumowski, P., Marfenina, O.E., Dunoyer-Geindre, S., Georgen, F., Roch-Susuki, R., Gallaz, L. and Latge, J.P., 1998. Mycological control and surveillance of biological waste and compost. Med Mycol, 36 Suppl 1:137-145.
6. Burnie, J.P., Carter, T.L., Hodgetts, S.J. and Matthews, R.C., 2006. Fungal heatshock proteins in human disease. FEMS microbiology reviews, 30:53-88.
7. Cerqueira, G.C., Arnaud, M.B., Inglis, D.O., Skrzypek, M.S., Binkley, G., Simison, M., Miyasato, S.R., Binkley, J., Orvis, J., Shah, P., Wymore, F., Sherlock, G. and Wortman, J.R., 2014. The aspergillus genome database: Multispecies curation and incorporation of rna-seq data to improve structural gene annotations. Nucleic Acids Res, 42:D705-710.
8. Chenna, R., Sugawara, H., Koike, T., Lopez, R., Gibson, T.J., Higgins, D.G. and Thompson, J.D., 2003. Multiple sequence alignment with the clustal series of programs. Nucleic acids research, 31:3497-3500.
9. Craig, E., Ziegelhoffer, T., Nelson, J., Laloraya, S. and Halladay, J., 1995. Complex multigene family of functionally distinct hsp70s of yeast. Cold Spring Harb Symp Quant Biol, 60:441-449.
10. Dagenais, T.R. and Keller, N.P., 2009. Pathogenesis of aspergillus fumigatus in invasive aspergillosis. Clinical microbiology reviews, 22:447-465.
11. Daugaard, M., Rohde, M. and Jaattela, M., 2007. The heat shock protein 70 family: Highly homologous proteins with overlapping and distinct functions. FEBS Lett, 581:3702-3710.
12. Fassler, J.S. and West, A.H., 2011. Fungal skn7 stress responses and their relationship to virulence. Eukaryot Cell, 10:156-167.
13. Felsenstein, J., 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 783-791.
14. Goodley, J., Clayton, Y. and Hay, R., 1994. Environmental sampling for aspergilli during building construction on a hospital site. Journal of Hospital Infection, 26:27-35.
15. Hesterkamp, T. and Bukau, B., 1998. Role of the dnak and hsca homologs of hsp70 chaperones in protein folding in e.Coli. EMBO J, 17:4818-4828.
16. Holder, M. and Lewis, P.O., 2003. Phylogeny estimation: Traditional and bayesian approaches. Nature reviews genetics, 4:275-284.
17. Holder, M. and Lewis, P.O., 2003. Phylogeny estimation: Traditional and bayesian approaches. Nat Rev Genet, 4:275-284.
18. Hospenthal, D., Kwon-Chung, K. and Bennett, J., 1998. Concentrations of airborne aspergillus compared to the incidence of invasive aspergillosis: Lack of correlation. Medical mycology, 36:165-168. 
19. Itoh, T., Matsuda, H. and Mori, H., 1999. Phylogenetic analysis of the third hsp70 homolog in escherichia coli; a novel member of the hsc66 subfamily and its possible co-chaperone. DNA Res, 6:299-305.
20. Jolly, C. and Morimoto, R.I., 2000. Role of the heat shock response and molecular chaperones in oncogenesis and cell death. Journal of the National Cancer Institute, 92:1564-1572.
21. Jung, K.W., Kang, H.A. and Bahn, Y.S., 2013. Essential roles of the kar2/bip molecular chaperone downstream of the upr pathway in cryptococcus neoformans. PLoS One, 8:e58956.
22. Kimura, M., 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of molecular evolution, 16:111-120.
23. Latgé, J.-P., 1999. Aspergillus fumigatus and aspergillosis. Clinical microbiology reviews, 12:310-350.
24. Latge, J.P., 1999. Aspergillus fumigatus and aspergillosis. Clin Microbiol Rev, 12:310-350.
25. Lelivelt, M.J. and Kawula, T.H., 1995. Hsc66, an hsp70 homolog in escherichia coli, is induced by cold shock but not by heat shock. J Bacteriol, 177:4900-4907.
26. Lin, S.-J., Schranz, J. and Teutsch, S.M., 2001. Aspergillosis case-fatality rate: Systematic review of the literature. Clinical Infectious Diseases, 32:358-366.
27. Mayer, M.P. and Bukau, B., 2005. Hsp70 chaperones: Cellular functions and molecular mechanism. Cell Mol Life Sci, 62:670-684.
28. Noe, L. and Kucherov, G., 2005. Yass: Enhancing the sensitivity of DNA similarity search. Nucleic Acids Res, 33:W540-543.
29. Nollen, E.A. and Morimoto, R.I., 2002. Chaperoning signaling pathways: Molecular
chaperones as stress-sensing 'heat shock' proteins. J Cell Sci, 115:2809-2816.
30. Normington, K., Kohno, K., Kozutsumi, Y., Gething, M.J. and Sambrook, J., 1989. S. Cerevisiae encodes an essential protein homologous in sequence and function to mammalian bip. Cell, 57:1223-1236.
31. O’Gorman, C.M., 2011. Airborneaspergillus fumigatus conidia: A risk factor for aspergillosis. Fungal biology reviews, 25:151-157.
32. Sharma, D. and Masison, D.C., 2009. Hsp70 structure, function, regulation and influence on yeast prions. Protein Pept Lett, 16:571-581.
33. Spicer, W.J., 2008. Clinical microbiology and infectious diseases: An illustrated colour text. Elsevier Health Sciences,
34. St-GERMAIN, G. and SUMMERBELL, R., 2003. Identifying filamentous fungi: A clinical laboratory handbook. Revista do Instituto de Medicina Tropical de São Paulo, 45:152-152.
35. Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S., 2011. Mega5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular biology and evolution, 28:2731-2739.
36. Yang, Z. and Bielawski, J.P., 2000. Statistical methods for detecting molecular adaptation. Trends in Ecology & Evolution, 15:496-503.
37. Zhang, Y., Ohashi, N. and Rikihisa, Y., 1998. Cloning of the heat shock protein 70 (hsp70) gene of ehrlichia sennetsu and differential expression of hsp70 and hsp60 mrna after temperature upshift. Infection and immunity, 66:3106-3112.
38. Zugel, U. and Kaufmann, S.H., 1999. Role of heat shock proteins in protection from and pathogenesis of infectious diseases. Clin Microbiol Rev, 12:19-39.
Journal of Mycology Research
Volume 2, Issue 2 - Serial Number 2
Summer & Autumn
2015
Pages 85-96

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