Role of denture-wearing on colonization and antifungal resistance of oral Candida albicans isolates in healthy people

Document Type : Original Articles


1 Mycology Research Center , Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Faculty of Dentistry, Tabriz University of Medical Science, Tabriz, Iran

3 Faculty of Nursing and Midwifery, Tabriz University of Medical Science, Tabriz, Iran


The primary focus of the present study was to evaluate the occurrence of Candida albicans isolates
in the oral cavity and its probable correlation with dental implant applications and prosthesis. We
collected oral swabs from patients who had attended private dentistry clinics, followed by stringent
and controlled antifungal susceptibility testing and calculation of colony forming units (CFUs).
Amphotericin B, Fluconazole and Itraconazole were three antifungals tested by CLSI M27-A2 broth
microdilution protocol. The MIC ranges for three tested antifungals in Candida albicans isolates
were obtained as 0.0625-1, 0.125-16 and 0.0313-0.5 μg/ml, respectively. Additionally, in non-
Candida albicans isolates, MIC ranges for three antifungals were achieved as 0.25-1, 0.125-16 and
0.0313-0.125 μg/ml, respectively. MIC50 values of both tested azoles in the Candida albicansgroup
were higher than related values in the non-Candida albicans group. Moreover, CFU counts for
denture-acquired people were higher than for participants not wearing denture applications,
indicating the proposal that the surface of dentures or any other synthetic implants in the oral cavity
may result in providing an appropriate environment for the colonization of yeasts.